genome copy number variation and genome-wide profiling of DNA-binding proteins 

irvinepharma

Abiraterone-prednisone confirmed superiority over prednisone alone with respect to time to initiation of cytotoxic chemotherapy, opiate use for cancer-related ache, prostate-specific antigen development

Abiraterone acetate, an androgen biosynthesis inhibitor, improves total survival in sufferers with metastatic castration-resistant prostate most cancers after chemotherapy. We evaluated this agent in sufferers who had not acquired earlier chemotherapy.

METHODS

On this double-blind research, we randomly assigned 1088 sufferers to obtain abiraterone acetate (1000 mg) plus prednisone (5 mg twice each day) or placebo plus prednisone. The coprimary finish factors had been radiographic progression-free survival and total survival.

RESULTS

The research was unblinded after a deliberate interim evaluation that was carried out after 43% of the anticipated deaths had occurred. The median radiographic progression-free survival was 16.5 months with abiraterone-prednisone and eight.three months with prednisone alone (hazard ratio for abiraterone-prednisone vs. prednisone alone, 0.53; 95% confidence interval [CI], 0.45 to 0.62; P<0.001). Over a median follow-up interval of 22.2 months, total survival was improved with abiraterone-prednisone (median not reached, vs. 27.2 months for prednisone alone; hazard ratio, 0.75; 95% CI, 0.61 to 0.93; P=0.01) however didn’t cross the efficacy boundary.

Abiraterone-prednisone confirmed superiority over prednisone alone with respect to time to initiation of cytotoxic chemotherapy, opiate use for cancer-related ache, prostate-specific antigen development, and decline in efficiency standing. Grade three or four mineralocorticoid-related hostile occasions and abnormalities on liver-function testing had been extra widespread with abiraterone-prednisone.

CONCLUSIONS

Abiraterone improved radiographic progression-free survival, confirmed a development towards improved total survival, and considerably delayed medical decline and initiation of chemotherapy in sufferers with metastatic castration-resistant prostate most cancers. (Funded by Janssen Analysis and Growth, previously Cougar Biotechnology; ClinicalTrials.gov quantity, NCT00887198.).

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PI/RNAse

PI/RNASE 200 test
EUR 173.5

50 ML AMPHOTERICIN B, LIQUID 250 µG/ML SOLUBILIZED

30-003-CF 50 mL/pk
EUR 81
Description: Media Catalog; Cell Culture Reagents

50 ML KANAMYCIN SULFATE, LIQUID, 5,000 µG/ML SOLUTION

30-006-CF 50 mL/pk
EUR 98
Description: Media Catalog; Cell Culture Reagents

PI 828

B7198-1 1 mg
EUR 126

PI-103

1728-1
EUR 153

Amphotericin B (Fungizone) Sol, 250 µg/mL

CCM1120-100 100 mL
EUR 110.23
  • Product category: Culture Media

100 ML ANTIBIOTIC-ANTIMYCOTIC SOLUTION, 10,000 I.U. PENICILLIN (PER ML) 10,000 µG/ML STREPTOMYCIN 25 µG/ML AMPHOTERICIN WITH 8.5 G/L NACI

30-004-CI 100 mL/pk
EUR 103
Description: Media Catalog; Cell Culture Reagents

Human Proinsulin (PI) ELISA Kit

DLR-PI-Hu-48T 48T
EUR 479
  • Should the Human Proinsulin (PI) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Proinsulin (PI) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Proinsulin (PI) ELISA Kit

DLR-PI-Hu-96T 96T
EUR 621
  • Should the Human Proinsulin (PI) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Proinsulin (PI) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Porcine Proinsulin (PI) ELISA Kit

DLR-PI-p-48T 48T
EUR 547
  • Should the Porcine Proinsulin (PI) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Porcine Proinsulin (PI) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Porcine Proinsulin (PI) ELISA Kit

DLR-PI-p-96T 96T
EUR 715
  • Should the Porcine Proinsulin (PI) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Porcine Proinsulin (PI) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Proinsulin (PI) ELISA Kit

RD-PI-Hu-48Tests 48 Tests
EUR 478

Human Proinsulin (PI) ELISA Kit

RD-PI-Hu-96Tests 96 Tests
EUR 662

Porcine Proinsulin (PI) ELISA Kit

RD-PI-p-48Tests 48 Tests
EUR 555

Porcine Proinsulin (PI) ELISA Kit

RD-PI-p-96Tests 96 Tests
EUR 771

Human Proinsulin (PI) ELISA Kit

RDR-PI-Hu-48Tests 48 Tests
EUR 500

Human Proinsulin (PI) ELISA Kit

RDR-PI-Hu-96Tests 96 Tests
EUR 692

Porcine Proinsulin (PI) ELISA Kit

RDR-PI-p-48Tests 48 Tests
EUR 580

Porcine Proinsulin (PI) ELISA Kit

RDR-PI-p-96Tests 96 Tests
EUR 807

dNTP Mix (1 ml)

9002-1
EUR 153

Fluorguard Antifade Mounting Medium

F001 1 ml
EUR 86

Penicillin-Streptomycin 100X Solution, 10, 000 Units/mL, 10, 000 µg/mL

CCM1133-100 100 mL
EUR 64.05
  • Product category: Culture Media

100 ML PENICILLIN-STREPTOMYCIN SOLUTION, 50X, 5,000 I.U. PENICILLIN (PER ML) 5,000 µG/ML STREPTOMYCIN

30-001-CI 100 mL/pk
EUR 82
Description: Media Catalog; Cell Culture Reagents

ML-211

C3647-1 1 mg
EUR 112
Description: IC50: LYPLA1 (17 nM) and the related LYPLA2 (30 nM)ML-211 is a dual inhibitor of LYPLA1 and the related LYPLA2.Lysophospholipase 1 (LYPLA1), a protein palmitoyl thioesterase, is responsible for depalmitoylation of the oncogene HRas.

ML-162

B2304-1
EUR 142

ML-356

C3354-1 1 mg
EUR 126
Description: ML-356 is an inhibitor of the thioesterase domain of fatty acid synthase with IC50 value of 334 nM.Fatty acid synthase (FAS) is a multi-enzyme protein that catalyzes fatty acid synthesis, fatty acids are related to energy production and storage, cellular structure ,hormones biosynthesis

ML-324

2763-1
EUR 131

ML-277

B2730-1 1 mg
EUR 115

HIV Infection Enhancer (Medium) (1 ml)

H901-1 NULL
EUR 0

100 ML PENICILLIN-STREPTOMYCIN-L-GLUTAMINE, 100X, 10,000 I.U. PENCILLIN (PER ML) 10,000 µG/ML STREPTOMYCIN 29.2 MG/ML L-GLUTAMINE

30-009-CI 100 mL/pk
EUR 80
Description: Media Catalog; Cell Culture Reagents

Anti-GST3/GST pi/Gstp1 Antibody

A00394-1 100ug/vial
EUR 334

StemBoost? SMAD Signaling Inhibitor Cocktail Set (1000X), Sterile-Filtered

K877-1-ML
EUR 153

Camptothecin (2 mg/ml)

1039-1
EUR 142

Kanamycin solution (50mg/ml)

MN800A-1 20 ml
EUR 127
  • Category: Minicircle Technology

CF633 Goat Anti-Mouse IgG1 (γ1), 2mg/mL

20250-1 50uL
EUR 149
Description: Minimum order quantity: 1 unit of 50uL

CF640R Goat Anti-Mouse IgG1 (γ1), 2mg/mL

20251-1 50uL
EUR 149
Description: Minimum order quantity: 1 unit of 50uL

CF647 Goat Anti-Mouse IgG1 (γ1), 2mg/mL

20252-1 50uL
EUR 149
Description: Minimum order quantity: 1 unit of 50uL

CF680 Goat Anti-Mouse IgG1 (γ1), 2mg/mL

20253-1 50uL
EUR 161
Description: Minimum order quantity: 1 unit of 50uL

CF770 Goat Anti-Mouse IgG1 (γ1), 2mg/mL

20254-1 50uL
EUR 161
Description: Minimum order quantity: 1 unit of 50uL

CF543 Goat Anti-Mouse IgG1 (γ1), 2mg/mL

20325-1 50uL
EUR 149
Description: Minimum order quantity: 1 unit of 50uL

CF350 Goat Anti-Mouse IgG1 (γ1), 2mg/mL

20245-1 50uL
EUR 149
Description: Minimum order quantity: 1 unit of 50uL

CF488A Goat Anti-Mouse IgG1 (γ1), 2mg/mL

20246-1 50uL
EUR 149
Description: Minimum order quantity: 1 unit of 50uL

CF555 Goat Anti-Mouse IgG1 (γ1), 2mg/mL

20247-1 50uL
EUR 149
Description: Minimum order quantity: 1 unit of 50uL

CF568 Goat Anti-Mouse IgG1 (γ1), 2mg/mL

20248-1 50uL
EUR 149
Description: Minimum order quantity: 1 unit of 50uL

CF594 Goat Anti-Mouse IgG1 (γ1), 2mg/mL

20249-1 50uL
EUR 149
Description: Minimum order quantity: 1 unit of 50uL

NATtrol Respiratory Pathogen Panel -1 (6 x 0.25 mL)

NATRPP-1 6 x 0.25 mL
EUR 464.96
  • What is the product classification?
  • NATtrol Respiratory Pathogen Panel -1 is marked as RUO.
Description: Please contact Gentaur in order to receive the datasheet of the product.

pI

EF007077 96 Tests
EUR 689

PI

abx290031-400tests 400 tests
EUR 258
  • Shipped within 2-3 weeks.

AXYPREP MAG DYECLEAN KIT- 1 ML - (SAMPLE SIZE)

MAG-DYECL-1 1/pk
EUR 75
Description: Bioscience Mag Beads; Magnetic Dye

Arachidonic Acid 5mg/ml

ABP-ARA-1 3 x 0,5ml vials
EUR 57

PI/ Rat PI ELISA Kit

ELA-E0379r 96 Tests
EUR 886

ExoQuick exosome precipitation solution (20 ml)

EXOQ20A-1 20 ml
EUR 1179
  • Category: Exosomes

ExoQuick exosome precipitation solution (5 ml)

EXOQ5A-1 5 ml
EUR 400
  • Category: Exosomes

AAVancedTM Concentration Reagent (100 ml aliquot)

AAV100A-1 100 ml
EUR 521
  • Category: Vectors

AAVancedTM Concentration Reagent (250 ml aliquot)

AAV110A-1 250 ml
EUR 999
  • Category: Vectors

GSTP2 Glutathione S-Transferase pi 2 Mouse Recombinant Protein

PROTP46425-1 Regular: 20ug
EUR 317
Description: GSTP2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 210 amino acids (1-210 a.a.) and having a molecular mass of 23.5kDa.; The GSTP2 is purified by proprietary chromatographic techniques.

Random Hexamers 100 µg

26-4000-03 100 ug
EUR 125
Description:

Random Primers are a mixture of oligonucleotides representing all possible sequence for that size. Random Primers can be used to prime synthesis in oligo-labeling similar to using hexamers (1,2) and cDNA synthesis. Random prime labeling yields high specific activity labeled DNA probe which can be used for all southern, northern and in situ hybridization studies. Random Primers can be also used similar to using hexamers in cDNA synthesis in combination with oligo d(T) to yield more 5' end cDNA sequence. Recently random primers have been used to detect DNA polymorphism. These polymorphisms, simply detected as DNA segments which amplify from one parent but not the other, are inherited in a Mendelian fashion and can be used to construct genetic maps in a variety of species. The authors suggested that these polymorphisms be called RAPD (pronounced RAPID) makers, after Random Amplified Polymorphic DNA (3).

References 1. Feinberg, A.P. & Vogelstein, B. (1983) Anal. Biochem. 132:6-13. 2. Feinberg, A.P. & Vogelstein, B. (1984) Anal. Biochem. 137:266-267. 3. Williams J. G., Kubelik A.R., Livak K.J., Rafalski J.A. & Tingey S.V. (1990) Nucleic Acid Res. 18(22):6531-5.

AXYPREP FRAGMENTSELECT-I KIT- 1 ML - (SAMPLE SIZE)

MAG-FRAG-I-1 1/pk
EUR 64
Description: Bioscience Mag Beads; Magnetic Fragment Select

ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL)

EXOQ5TM-1 75 reactions
EUR 843
  • Category: Exosomes

PI-3065

B1674-25
EUR 506

PI-3065

B1674-5
EUR 158

PI-3065

B4660-10 10 mg
EUR 224
Description: PI-3065 is a small molecule and selective inhibitor of p110?kinase with an Ki value of 1.5nM and IC50 value of 5nM [1].PI-3065 has been reported to selectively inhibit p110?kinase with Ki values of 15nM, 110nM, 130nM and 940nM

PI-3065

B4660-100 100 mg
EUR 792
Description: PI-3065 is a small molecule and selective inhibitor of p110?kinase with an Ki value of 1.5nM and IC50 value of 5nM [1].PI-3065 has been reported to selectively inhibit p110?kinase with Ki values of 15nM, 110nM, 130nM and 940nM

PI-3065

B4660-5 5 mg
EUR 166
Description: PI-3065 is a small molecule and selective inhibitor of p110?kinase with an Ki value of 1.5nM and IC50 value of 5nM [1].PI-3065 has been reported to selectively inhibit p110?kinase with Ki values of 15nM, 110nM, 130nM and 940nM

PI-3065

B4660-5.1 10 mM (in 1mL DMSO)
EUR 177
Description: PI-3065 is a small molecule and selective inhibitor of p110?kinase with an Ki value of 1.5nM and IC50 value of 5nM [1].PI-3065 has been reported to selectively inhibit p110?kinase with Ki values of 15nM, 110nM, 130nM and 940nM

PI-3065

B4660-50 50 mg
EUR 572
Description: PI-3065 is a small molecule and selective inhibitor of p110?kinase with an Ki value of 1.5nM and IC50 value of 5nM [1].PI-3065 has been reported to selectively inhibit p110?kinase with Ki values of 15nM, 110nM, 130nM and 940nM

PI-1840

B4755-10 10 mg
EUR 184
Description: PI-1840 is a potent and selective inhibitor of proteasome with IC50 value of 27 nM for chymotrypsinlike (CT-L) activity [1]. The 26S proteasome consists of a 19S regulatory particle (RP) and a 20S core particle.

PI-1840

B4755-50 50 mg
EUR 522
Description: PI-1840 is a potent and selective inhibitor of proteasome with IC50 value of 27 nM for chymotrypsinlike (CT-L) activity [1]. The 26S proteasome consists of a 19S regulatory particle (RP) and a 20S core particle.

PI 828

B7198-10 10 mg
EUR 318

PI 828

B7198-5 5 mg
EUR 203

PI-103

E1KS1038 5mg
EUR 325

PI-103

HY-10115 100mg
EUR 546

PI-828

HY-108606 1mg
EUR 142

PI-3065

HY-12235 10mM/1mL
EUR 204

PI-1840

HY-12286 50mg
EUR 587

PI / RNAse

abx290029-200tests 200 tests
EUR 272
  • Shipped within 2-3 weeks.

PI-103

A2067-25 25 mg
EUR 328
Description: PI-103 is a potent and selective inhibitor of class I PI3K, mTOR and DNA-PK with IC50 values of 2, 3, 3, 15, 30 and 23 nM for p110?, p110?, p110?, p110?, mTOR and DNA-PK, respectively [1].

PI-103

A2067-5 5 mg
EUR 147
Description: PI-103 is a potent and selective inhibitor of class I PI3K, mTOR and DNA-PK with IC50 values of 2, 3, 3, 15, 30 and 23 nM for p110?, p110?, p110?, p110?, mTOR and DNA-PK, respectively [1].

PI-103

A2067-5.1 10 mM (in 1mL DMSO)
EUR 154
Description: PI-103 is a potent and selective inhibitor of class I PI3K, mTOR and DNA-PK with IC50 values of 2, 3, 3, 15, 30 and 23 nM for p110?, p110?, p110?, p110?, mTOR and DNA-PK, respectively [1].

PI-103

A2067-S Evaluation Sample
EUR 81
Description: PI-103 is a potent and selective inhibitor of class I PI3K, mTOR and DNA-PK with IC50 values of 2, 3, 3, 15, 30 and 23 nM for p110?, p110?, p110?, p110?, mTOR and DNA-PK, respectively [1].

PI-103

1728-5
EUR 419

PI-103

1728-50 Ask for price

BrightGreen miRNA qPCR MasterMix-Low ROX

MasterMix-mL 4 x 1.25 ml for 500 reactions
EUR 151

Rat Monoclonal Anti-Mouse CD4 Pur. Low Endotoxin (Clone YTS 191.1) (rat IgG2b)

MCD004-ML 100 ug
EUR 408

Rat Monoclonal Anti-Mouse CD25 , Pur. Low Endotoxin (Clone PC61.5.3) (rat IgG1)

MCD025-ML 100 ug
EUR 408

Steady-Luc Firefly HTS Assay Kit (12 ml)

30028-1 1KIT
EUR 139
Description: Minimum order quantity: 1 unit of 1KIT

PEG-it Virus Precipitation Solution (250 mL aliquot)

LV825A-1 250 ml
EUR 777
  • Category: Lentiviral Technology

PEG-it Virus Precipitation Solution (100 mL aliquot)

LV810A-1 100 ml
EUR 382
  • Category: Lentiviral Technology

Water-soluble Cumate solution (300mg/ml, 10,000x), 500ul

QM150A-1 500 ul
EUR 164
  • Category: Lentiviral Technology

AXYPREP MAG PCR NORMALIZER KIT- 1 ML - (SAMPLE SIZE)

MAG-PCR-NM-1 1/pk
EUR 73
Description: Bioscience Mag Beads; Magnetic PCR Normaliser

 RNA templates, assay designs and protocols, in addition to inappropriate information normalization and inconsistent information evaluation, are extensively identified but in addition extensively disregarded 

The actual-time reverse transcription polymerase chain response (RT-qPCR) addresses the evident requirement for quantitative information evaluation in molecular medication, biotechnology, microbiology and diagnostics and has change into the tactic of alternative for the quantification of mRNA. Though it’s typically described as a “gold” customary, it’s removed from being a regular assay. The numerous issues attributable to variability of RNA templates, assay designs and protocols, in addition to inappropriate information normalization and inconsistent information evaluation, are extensively identified but in addition extensively disregarded.

As a primary step in the direction of standardization, we describe a sequence of RT-qPCR protocols that illustrate the important technical steps required to generate quantitative information which might be dependable and reproducible.

We want to emphasize, nevertheless, that RT-qPCR information represent solely a snapshot of data relating to the amount of a given transcript in a cell or tissue. Any evaluation of the organic penalties of variable mRNA ranges should embrace extra data relating to regulatory RNAs, protein ranges and protein exercise. The whole protocol described right here, encompassing all phases from preliminary assay design to dependable qPCR information evaluation, requires roughly 15 h.

 

non-gene-expression research, together with examination of genome copy quantity variation and genome-wide profiling of DNA-binding proteins 

A decade in the past, the Gene Expression Omnibus (GEO) database was established on the Nationwide Middle for Biotechnology Data (NCBI). The unique goal of GEO was to function a public repository for high-throughput gene expression information generated largely by microarray know-how. Nonetheless, the analysis group rapidly utilized microarrays to non-gene-expression research, together with examination of genome copy quantity variation and genome-wide profiling of DNA-binding proteins. As a result of the GEO database was designed with a versatile construction, it was attainable to rapidly adapt the repository to retailer these information varieties. Extra not too long ago, because the microarray group switches to next-generation sequencing applied sciences, GEO has once more tailored to host these information units.

In the present day, GEO shops over 20,000 microarray- and sequence-based purposeful genomics research, and continues to deal with the vast majority of direct high-throughput information submissions from the analysis group.

A number of mechanisms are supplied to assist customers successfully search, browse, obtain and visualize the info on the stage of particular person genes or total research. This paper describes current database enhancements, together with new search and information illustration instruments, in addition to a quick assessment of how the group makes use of GEO information. GEO is freely accessible at

beta-glucuronidase (GUS), artificial inexperienced fluorescent protein with S65T mutation (sGFP), luciferase (LUC), enhanced yellow fluorescent protein (EYFP), and enhanced cyan fluorescent protein (ECFP) 

We developed a brand new sequence of binary vectors helpful for Gateway cloning to facilitate transgenic experiments in plant biotechnology. The brand new system, Gateway Binary Vectors (pGWBs) realized environment friendly cloning, constitutive expression utilizing the cauliflower mosaic virus (CaMV) 35S promoter and the development of fusion genes by easy clonase response with an entry clone.

The reporters employable on this system are beta-glucuronidase (GUS), artificial inexperienced fluorescent protein with S65T mutation (sGFP), luciferase (LUC), enhanced yellow fluorescent protein (EYFP), and enhanced cyan fluorescent protein (ECFP). The tags accessible are 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7-epitope, and tandem affinity purification (TAP). In complete, 13 sorts of reporter or tag had been organized and had been virtually relevant to each N- and C-fusions.

The pGWBs could possibly be used for a lot of functions, equivalent to promoter::reporter evaluation, statement of subcellular localization by the expression of proteins fused to a reporter or tag, and evaluation of protein-protein interplay by copurification and immunodetection experiments.

The pGWBs had been constructed with modified pBI101 containing a CaMV35S promoter-driven hygromycin phosphotransferase (HPT) gene because the second choice marker. We additionally constructed pGWBs with the marker HPT pushed by the nopaline synthase promoter. Through the use of the pGWB system, the expression of tagged proteins, and the localization of GFP-fused proteins had been simply analyzed.

Furthermore, tissue-specific and inducible gene expression utilizing a promoter was additionally monitored with pGWBs. It’s anticipated that, the pGWB system will function a robust instrument for plasmid development in plant analysis.

 

Nationwide Middle for Biotechnology Data (NCBI) is the biggest public repository for high-throughput gene expression information 

The Gene Expression Omnibus (GEO) on the Nationwide Middle for Biotechnology Data (NCBI) is the biggest public repository for high-throughput gene expression information. Moreover, GEO hosts different classes of high-throughput purposeful genomic information, together with people who study genome copy quantity variations, chromatin construction, methylation standing and transcription issue binding. These information are generated by the analysis group utilizing high-throughput applied sciences like microarrays and, extra not too long ago, next-generation sequencing.

The database has a versatile infrastructure that may seize totally annotated uncooked and processed information, enabling compliance with main community-derived scientific reporting requirements equivalent to ‘Minimal Data A few Microarray Experiment’ (MIAME). Along with serving as a centralized information storage hub, GEO provides many instruments and options that enable customers to successfully discover, analyze and obtain expression information from each gene-centric and experiment-centric views. This text summarizes the GEO repository construction, content material and working procedures, in addition to not too long ago launched information mining options.

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processes for metabolic and gene expression profiling, these platforms constitute

metal-oxide-semiconductor (CMOS) course of, permits for low-cost, large-scale manufacturing and scaling of the machine to larger densities and bigger array sizes. The seminal significance of DNA sequencing to the life sciences, biotechnology and drugs has pushed the seek for extra scalable and lower-cost options. Right here we describe a DNA sequencing know-how during which scalable, low-cost semiconductor manufacturing […]

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